diff --git a/samples/bluetooth/peripheral_uart/boards/nrf5340dk_nrf5340_cpuapp.overlay b/samples/bluetooth/peripheral_uart/boards/nrf5340dk_nrf5340_cpuapp.overlay
new file mode 100644
index 000000000..dae8a491a
--- /dev/null
+++ b/samples/bluetooth/peripheral_uart/boards/nrf5340dk_nrf5340_cpuapp.overlay
@@ -0,0 +1,33 @@
+
+/delete-node/ &boot_partition;
+/delete-node/ &slot0_partition;
+/delete-node/ &slot0_ns_partition;
+/delete-node/ &slot1_partition;
+/delete-node/ &slot1_ns_partition;
+
+&flash0 {
+	partitions {
+		compatible = "fixed-partitions";
+
+		boot_partition: partition@0 {
+			label = "mcuboot";
+			reg = <0x00000000 0x00020000>;
+		};
+		slot0_partition: partition@20000 {
+			label = "image-0";
+            reg = <0x00020000 0x38000>;
+		};
+		slot0_ns_partition: partition@58000 {
+			label = "image-0-nonsecure";
+            reg = <0x00058000 0x30000>;
+		};
+		slot1_partition: partition@88000 {
+			label = "image-1";
+            reg = <0x00088000 0x38000>;
+		};
+		slot1_ns_partition: partition@c0000 {
+			label = "image-1-nonsecure";
+            reg = <0x000c0000 0x30000>;
+		};
+	};
+};
\ No newline at end of file
diff --git a/samples/bluetooth/peripheral_uart/child_image/mcuboot.conf b/samples/bluetooth/peripheral_uart/child_image/mcuboot.conf
new file mode 100644
index 000000000..ca2a9d728
--- /dev/null
+++ b/samples/bluetooth/peripheral_uart/child_image/mcuboot.conf
@@ -0,0 +1,31 @@
+CONFIG_LOG=y
+CONFIG_LOG_BACKEND_UART=y
+# The build won't fit on the partition allocated for it without size
+# optimizations.
+CONFIG_SIZE_OPTIMIZATIONS=y
+CONFIG_PM_PARTITION_SIZE_MCUBOOT=0x20000
+CONFIG_PCD=y
+
+# Serial
+CONFIG_SERIAL=y
+CONFIG_UART_NRFX=y
+CONFIG_UART_INTERRUPT_DRIVEN=y
+CONFIG_UART_LINE_CTRL=y
+
+# MCUBoot serial
+CONFIG_GPIO=y
+CONFIG_MCUBOOT_SERIAL=y
+CONFIG_BOOT_SERIAL_CDC_ACM=y
+CONFIG_BOOT_SERIAL_DETECT_PORT="GPIO_0"
+CONFIG_BOOT_SERIAL_DETECT_PIN=23
+
+# Required by USB
+CONFIG_MULTITHREADING=y
+
+# USB
+CONFIG_USB=y
+CONFIG_USB_DEVICE_STACK=y
+CONFIG_USB_DEVICE_PRODUCT="MCUBOOT"
+CONFIG_USB_CDC_ACM=y
+CONFIG_USB_COMPOSITE_DEVICE=n
+CONFIG_USB_MASS_STORAGE=n
diff --git a/samples/bluetooth/peripheral_uart/prj.conf b/samples/bluetooth/peripheral_uart/prj.conf
index cd6df0664..418bd9489 100644
--- a/samples/bluetooth/peripheral_uart/prj.conf
+++ b/samples/bluetooth/peripheral_uart/prj.conf
@@ -4,6 +4,8 @@
 # SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
 #
 
+CONFIG_BOOTLOADER_MCUBOOT=y
+
 # Enable the UART driver
 CONFIG_UART_ASYNC_API=y
 CONFIG_NRFX_UARTE0=y