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Data is not transmitted when changing the pin setting of Tx of UART of nRF52840

Hello.
I am testing the sending and receiving of UART using nRF 52840-DK. If you change the UART pin settings as follows, you can receive but you can not send.
It is confirmed that the waveform of the transmission data is not displayed on the oscilloscope.
Please tell me the countermeasure method.

Tx: P 0.06 -> P 0.23
Rx: P 0.08 -> P 0.26

The sample code uses peripheral / uart.

nRF 52840
SDK v 15.0.0
gcc-arm-none-eabi-7-2018-q2-update-win32

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