Why can't my application control the p1.00 and p1.01 pins of 5340?

I am developing a project using nRF5340 to drive some peripherals. During debugging, I found that no matter how I executed the pull-up/pull-down commands, the p1.00 and p1.01 pins remained uncontrollable and always stayed in a high-level state. Why is this? I have attached my config file and overlay file.

5050.prj.conf

7026.nrf5340dk_nrf5340_cpuapp_ns.overlay

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